Figure 7.

Target validation of a hypothalamic AAV-BDNF vector in female Magel2-null mice

(A) Relative gene expression of hypothalamic tissue. (B) Immunoblotting of an HA tag as a proxy for BDNF expression; each lane depicts protein obtained from a unilateral hypothalamic dissection from an individual mouse. (C) Representative image of AAV-YFP fluorescence (ARC, arcuate nucleus of the hypothalamus; VMH, ventromedial hypothalamus; 3V, third ventricle). Data are means ± SEM. Sample size: WT YFP n = 6, Magel2-null YFP n = 6, Magel2-null BDNF n = 5–6. Plus sign (+) indicates p < 0.010; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.