Figure 1.

Cell Squeeze^® platform generates Antigen Carriers (AC) which exhibit rapid in vivo uptake. (A) Schematic of Cell Squeeze^® microfluidic platform for intracellular delivery of cargo to RBCs. (B) Unprocessed mouse RBCs and Ova-AF647 squeezed carriers (AC-Ova) with distinct SSC^low and SSC^hi populations (from single cell gate). Left: flow plots (SSC-H vs. FSC-A). Right: Percent SSC^low and SSC^hi populations. (C) Annexin V levels and Ova-AF647 delivery in unprocessed RBCs and AC-Ova. (D) Percent Ova-AF647 delivery to events in SSC^low and SSC^hi populations. (E) Percent annexin V positive events in unprocessed RBC or carrier groups. (F) In vivo clearance kinetics of untouched RBCs and EC from n = 2 mice in PBS group and n = 3 mice in each unprocessed RBC and EC group. n = 2 independent studies. ****P < 0.0001, one-way ANOVA.