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. 2022 Oct 3;13:1002163. doi: 10.3389/fimmu.2022.1002163

Figure 1.

Figure 1

With aging, the myeloid population expands and Zfp36 expression is downregulated. (A) Flow cytometry analysis of M-MDSC populations in bone marrow, spleen, and mesenteric lymph nodes from young and old mice. (A) The UMAP plot of cells isolated from bone marrow. (B) Flow cytometry analysis of PMN-MDSC populations in bone marrow, spleen, and mesenteric lymph nodes from young and old mice. (C) Flow cytometry analysis of macrophage populations in bone marrow, spleen, and mesenteric lymph nodes from young and old mice. (D) The UMAP plot of cells isolated from bone marrow from 3-month-old mice. (E) The UMAP plot of 1, 3, 18, 21, and 30-month datasets shows the distribution of each age group. (F) The UMAP plot of myeloid cells isolated from bone marrow. (G) The UMAP plot of 1, 3, 18, 21, and 30-month datasets shows the distribution of each age group. (H, I) The UMAP plot of 1, 3, 18, 21, and 30-month datasets shows the distribution of Zfp36 expression in myeloid cells of each age group. (J) Violin plots showing the expression of Zfp36 in myeloid cells of each age group. (K) Quantification of Zfp36 mRNA expression in M-MDSC populations of young and old mice based on bulk RNA sequencing. Comparisons were analyzed using unpaired t tests; data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ****P < 0.0001.