FIGURE 2.

Experimental overview and lung slice repopulation. H441 cells were seeded into wells with mounted DLS and exposed to mechanical stimuli for 1 or 4 days, after which samples were collected (A). Cell metabolism was evaluated after 1 and 4 days of culture by a resazurin-based reduction assay (B). CS: cyclic stretch (circles), ST: static culture (squares). Data points represent data from individual lung slice samples (n = 6 for both conditions at day 1, and n = 5 at day 4), horizontal lines show group median. Representative images of hematoxylin and eosin (H,E) stained repopulated lung slices after 1 and 4 days of culture, with (CS) or without (ST) cyclic stretch (C). Scale bars are 50 µm.