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. 2022 Oct 3;13:939390. doi: 10.3389/fmicb.2022.939390

Table 2.

Process parameters for production of biogenic ZnO NP as per Box-Behnken design.

Run Temp (°C) pH Time (min) Conc of Zinc nitrate (mM) Vol of Extract (mL) Absorbance
1 25 8 90 50 25 0.742
2 40 6 90 30 10 0.698
3 10 6 180 30 25 0.778
4 40 6 90 50 25 0.784
5 25 4 0 30 25 0.008
6 25 8 180 30 25 0.756
7 25 4 90 30 40 0.638
8 10 8 90 30 25 0.612
9 10 6 90 50 25 0.743
10 40 6 90 30 40 0.796
11 40 6 180 30 25 0.843
12 25 8 0 30 25 0.01
13 25 4 90 50 25 0.789
14 25 8 90 30 40 0.824
15 40 4 90 30 25 0.756
16 25 6 90 30 25 0.425
17 25 6 0 30 10 0.009
18 25 6 0 50 25 0.012
19 25 6 180 50 25 0.921
20 40 8 90 30 25 0.655
21 25 6 90 10 10 0.512
22 25 6 90 30 25 0.768
23 10 6 90 30 10 0.626
24 10 4 90 30 25 0.678
25 25 6 90 50 10 0.684
26 10 6 90 10 25 0.712
27 25 6 180 10 25 0.801
28 25 4 180 30 25 0.814
29 10 6 90 30 40 0.842
30 25 6 0 10 25 0.012
31 10 6 0 30 25 0.014
32 40 6 90 10 25 0.234
33 25 6 90 50 40 0.744
34 25 4 90 10 25 0.521
35 25 6 90 30 25 0.667
36 25 4 90 30 10 0.7
37 25 6 90 10 40 0.734
38 25 6 90 30 25 0.746
39 25 6 90 30 25 0.742
40 25 8 90 30 10 0.745
41 25 6 0 30 40 0.012
42 25 6 180 30 40 0.845
43 25 6 180 30 10 0.848
44 40 6 0 30 25 0.018
45 25 8 90 10 25 0.661
46 25 6 90 30 25 0.621