Table 2.
Process parameters for production of biogenic ZnO NP as per Box-Behnken design.
| Run | Temp (°C) | pH | Time (min) | Conc of Zinc nitrate (mM) | Vol of Extract (mL) | Absorbance |
|---|---|---|---|---|---|---|
| 1 | 25 | 8 | 90 | 50 | 25 | 0.742 |
| 2 | 40 | 6 | 90 | 30 | 10 | 0.698 |
| 3 | 10 | 6 | 180 | 30 | 25 | 0.778 |
| 4 | 40 | 6 | 90 | 50 | 25 | 0.784 |
| 5 | 25 | 4 | 0 | 30 | 25 | 0.008 |
| 6 | 25 | 8 | 180 | 30 | 25 | 0.756 |
| 7 | 25 | 4 | 90 | 30 | 40 | 0.638 |
| 8 | 10 | 8 | 90 | 30 | 25 | 0.612 |
| 9 | 10 | 6 | 90 | 50 | 25 | 0.743 |
| 10 | 40 | 6 | 90 | 30 | 40 | 0.796 |
| 11 | 40 | 6 | 180 | 30 | 25 | 0.843 |
| 12 | 25 | 8 | 0 | 30 | 25 | 0.01 |
| 13 | 25 | 4 | 90 | 50 | 25 | 0.789 |
| 14 | 25 | 8 | 90 | 30 | 40 | 0.824 |
| 15 | 40 | 4 | 90 | 30 | 25 | 0.756 |
| 16 | 25 | 6 | 90 | 30 | 25 | 0.425 |
| 17 | 25 | 6 | 0 | 30 | 10 | 0.009 |
| 18 | 25 | 6 | 0 | 50 | 25 | 0.012 |
| 19 | 25 | 6 | 180 | 50 | 25 | 0.921 |
| 20 | 40 | 8 | 90 | 30 | 25 | 0.655 |
| 21 | 25 | 6 | 90 | 10 | 10 | 0.512 |
| 22 | 25 | 6 | 90 | 30 | 25 | 0.768 |
| 23 | 10 | 6 | 90 | 30 | 10 | 0.626 |
| 24 | 10 | 4 | 90 | 30 | 25 | 0.678 |
| 25 | 25 | 6 | 90 | 50 | 10 | 0.684 |
| 26 | 10 | 6 | 90 | 10 | 25 | 0.712 |
| 27 | 25 | 6 | 180 | 10 | 25 | 0.801 |
| 28 | 25 | 4 | 180 | 30 | 25 | 0.814 |
| 29 | 10 | 6 | 90 | 30 | 40 | 0.842 |
| 30 | 25 | 6 | 0 | 10 | 25 | 0.012 |
| 31 | 10 | 6 | 0 | 30 | 25 | 0.014 |
| 32 | 40 | 6 | 90 | 10 | 25 | 0.234 |
| 33 | 25 | 6 | 90 | 50 | 40 | 0.744 |
| 34 | 25 | 4 | 90 | 10 | 25 | 0.521 |
| 35 | 25 | 6 | 90 | 30 | 25 | 0.667 |
| 36 | 25 | 4 | 90 | 30 | 10 | 0.7 |
| 37 | 25 | 6 | 90 | 10 | 40 | 0.734 |
| 38 | 25 | 6 | 90 | 30 | 25 | 0.746 |
| 39 | 25 | 6 | 90 | 30 | 25 | 0.742 |
| 40 | 25 | 8 | 90 | 30 | 10 | 0.745 |
| 41 | 25 | 6 | 0 | 30 | 40 | 0.012 |
| 42 | 25 | 6 | 180 | 30 | 40 | 0.845 |
| 43 | 25 | 6 | 180 | 30 | 10 | 0.848 |
| 44 | 40 | 6 | 0 | 30 | 25 | 0.018 |
| 45 | 25 | 8 | 90 | 10 | 25 | 0.661 |
| 46 | 25 | 6 | 90 | 30 | 25 | 0.621 |