FIGURE 4.

The effect of ZNF683 transfection on NK cell phenotype alterations. (A) Volcano plot depicts the upregulated (right panel) and downregulated genes (left panel) in MM‐derived ZNF683⁺ NK cells compared with these in healthy volunteers. (B) Violin plots show ZNF683 expression among NK subsets in BM/PB from healthy volunteers and MM patients. *P < .05, **P < .01, by Wilcoxon rank‐sum test. (C) NK cells were isolated from PB of healthy volunteers (n = 3), then they were transfected with ZNF683 overexpressing vectors (ZNF683‐OE) or empty vectors (ZNF683‐NC). Flow cytometry results demonstrate the effect of ZNF683 transfection on expression of inhibitory receptors LAG3, CTLA4, TIGIT, CD158b. (D) Flow cytometry result demonstrates the effect of ZNF683 transfection on expression of activating receptor NKG2D and NKp46. For C and D, *P < .05, **P < .01, ****P < .0001, by two‐tailed Student's t test