Figure 1.

Biochemical fractionation of endogenous Rab29. Post-nuclear supernatants of (A) mouse primary glial cultures, (C) rat tissues (brain, heart, lung, liver, kidney, and spleen), and (E) A549 cells were fractionated by ultracentrifugation into the cytosol and membrane fractions. Representative immunoblots of three independent experiments with the indicated antibodies are shown. DJ-1 was used as a representative cytosolic protein, whereas Na+/K+ ATPase α-1 subunit was used as a membrane protein in (A, C, and E). B and D, the band intensity of Rab10 and Rab29 was quantified, and the ratio of cytosolic Rab to total Rab (cytosol + membrane) was calculated. The circles, the bars, and the error bars in the graphs represent individual values, the mean values, and their standard errors, respectively. ∗∗p < 0.01, ∗∗∗p < 0.001. The p-values and statistical tests used are summarized in Table S1.