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. 2022 Sep 16;298(10):102499. doi: 10.1016/j.jbc.2022.102499

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Coimmunoprecipitation of GDI1/2 with Rab proteins. HA-empty, HA-Rab10, HA-Rab29 WT, HA-Rab29 C202S+C203S (CCSS), or HA-Rab29 D63A were overexpressed in HEK293A cells and immunoprecipitated using HA-agarose beads (the bottom panel). Coimmunoprecipitation of GDI1 and GDI2 with the overexpressed HA-tagged proteins was examined by immunoblotting (the second panel from the bottom). Equal levels of the expression of GDI1/2 as well as HA-tagged proteins were shown in the top panels (input). GDI, GDP-dissociation inhibitor.