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Fractionation of various Rab proteins depending on their hydrophobicity using Triton X-114. HEK293A and U2OS cells were lysed in a buffer containing Triton X-114. Cleared lysates (input) were heated to 37 °C to cause phase separation of Triton X-114. The aqueous and detergent phases were subjected to immunoblotting with the indicated antibodies. DJ-1 was used as a representative hydrophilic protein, whereas the transferrin receptor was used as a hydrophobic protein.
