Figure 8.

The effect of the knockout of RABGGTA and RABGGTB on the hydrophobicity of Rab10 and Rab29. HEK293A cells lacking RABGGTA or RABGGTB gene were fractionated using Triton X-114 (A–C, RABGGTA KO cells; D–F, RABGGTB KO cells). A, B, D, and E, representative immunoblots of three independent experiments with the indicated antibodies are shown. DJ-1 was used as a representative hydrophilic protein, whereas the transferrin receptor was used as a hydrophobic protein. C and F, the band intensities of the Rab proteins were quantified, and the ratio of Rab in the detergent phase to total Rab (aqueous + detergent) was calculated. The circles, the bars, and the error bars in the graphs represent individual values, the mean values, and their standard errors, respectively. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. The p-values and statistical tests used are summarized in Table S1.