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. 2022 Sep 13;298(10):102484. doi: 10.1016/j.jbc.2022.102484

Figure 4.

Figure 4

The Stub1/Chic2 complex interacts with Csf2rb and Chic2 and Stub1 KO reduce Csf2rb ubiquitination.A, Western blots of anti-V5 immunoprecipitation of STUB1-V5 and whole cell lysate for V5, Chic2, Csf2rb, and Vinculin from 32D Cas9 cells cultured in 0.01 ng/ml IL-3. B, Western blots of anti-V5 immunoprecipitation of V5-CHIC2 and whole cell lysate for V5, Stub1, Csf2rb, and Vinculin from 32D Cas9 cells cultured in 0.01 ng/ml IL-3. C, Western blots of anti-V5 immunoprecipitation of STUB1-V5 and whole cell lysate for V5, Csf2rb, and Vinculin from 32D Cas9 cells cultured in 5, 0.1, or 0.01 ng/ml IL-3. D, Western blots of anti-V5 immunoprecipitation of STUB1-V5 or STUB1 ΔTPR-V5 and whole cell lysate for V5, Csf2rb, Chic2, and Vinculin from 32D Cas9 cells cultured in 0.01 ng/ml IL-3. E, Western blots of anti-V5 immunoprecipitation of STUB1-V5 and whole cell lysate for V5, Csf2rb, Chic2, and Vinculin in 32D Cas9 cells with sgNT or sgChic2-1/2 cultured in 0.01 ng/ml IL-3. F, Western blots of anti-V5 immunoprecipitation of STUB1-V5 and whole cell lysate for V5, Csf2rb, and Vinculin from 32D Cas9 cells cultured in 5, 0.1, or 0.01 ng/ml IL-3. G, Western blots of anti-V5 immunoprecipitation of STUB1-V5 and whole cell lysate for V5, Csf2rb, Chic2, and Vinculin from 32D Cas9 cells cultured in 0.01 ng/ml IL-3 and treated with or without Ruxolitinib (100 nM) for 2 h.