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. 2022 Sep 13;298(10):102484. doi: 10.1016/j.jbc.2022.102484

Figure 5.

Figure 5

Stub1 and Chic2 KO lead to deceased ubiquitination of Csf2rb and blocks the effect of a lysosomal acidification inhibitor on Csf2rb stability.A, Western blots of TUBE immunoprecipitation and whole cell lysate for Csf2rb, Chic2, Stub1, and Vinculin as a loading control in 32D Cas9 cells with sgNT, sgChic2-1/2, or sgStub1-1/2 cultured in 0.01 ng/ml IL-3 and treated with 1 μM Bafilomycin A1 and 10 μM MG132 for 4 h. B, Western blots of Csf2rb and Vinculin in 32D cas9 cells with sgNT, sgStub1-1, or sgChic2-1 treated with DMSO or bafilomycin A1 (100 nM) for 16 h. DMSO, dimethyl sulfoxide.