Primary antibodies:
| Antibody | Use | Recognized species | Source | Catalog # | Species | Dilution |
|---|---|---|---|---|---|---|
| AKT | W | Mouse/human | CST | 4685 | Rabbit | 1:1000 |
| pS473-AKT | W | Mouse/human | CST | 4060 | Rabbit | 1:1000 |
| CSF2RB (IL3RB) | W | Mouse | Abcam | ab86743 | Rat | 1:1000 |
| CSF2RB (CD131) - PE | F | Mouse | BD Bioscience | 559920 | Rat | 1:200 |
| CHIC2 | W | Mouse/human | Sigma Aldrich | SAB2103335 | Rabbit | 1:1000 |
| ERK 1/2 | W | Mouse/human | CST | 4696 | Rabbit | 1:1000 |
| pT202/Y205-ERK 1/2 | W | Mouse/human | CST | 4370 | Rabbit | 1:1000 |
| IL3RA (CD123) | F | Mouse | BioLegend | 106005 | Rat | 1:200 |
| Rat IgG1,k isotype control | F | Mouse | BioLegend | 554685 | Rat | 1:200 |
| STAT5 | W | Mouse/human | CST | 94205 | Rabbit | 1:1000 |
| pY694-STAT5 | W | Mouse/human | CST | 4322 | Rabbit | 1:1000 |
| STUB1 (CHIP) | W | Mouse/human | CST | 2080 | Rabbit | 1:1000 |
| V5 tag | W | N/A | Abcam | ab27671 | Mouse | 1:1000 |
| V5 tag | W | N/A | CST | 13202 | Rabbit | 1:1000 |
| Vinculin | W | Mouse/human | Sigma Aldrich | V9131 | Mouse | 1:10,000 |