| Primary antibodies | ||||
|---|---|---|---|---|
| WB, Western Blotting; IF, Immunofluorescence; PLA, Proximity Ligation Assay | ||||
| Antibody | Species | Supplier | Cat # | Dilution |
| Synaptopodin | Rabbit | Synaptic Systems | 163002 |
WB 1:1,000 IF 1:600 |
| Beta‐actin | Mouse | Sigma‐Aldrich | A5316 | WB 1:5,000 |
| GFP | Mouse | Roche | 11867423001 | WB 1:5,000 |
| GFP | Mouse | Roche | 11814460001 | IF live 1:200 |
| GluA2 | Mouse | Synaptic Systems | 182411 | IF live 1:100 |
| GluA1 | Rabbit | Agrobio (Choquet) | 2144 clone G02141 | IF live 1:100 |
| VGLUT1 | Guinea Pig | Merck Millipore | AB5905 | IF 1:5,000 |
| MAP2 | Rabbit | Merck Millipore | AB5622 | IF 1:1,000 |
| MAP2 | Guinea Pig | Synaptic Systems | 188004 | IF 1:2,000 |
| PSD‐95 | Mouse | Merck Millipore | MA1‐046 | IF 1:500 |
| HA | Rat | Roche | 11867423001 | IF live 1:100 |
| Pan neuronal | Mouse | Merck Millipore | MAB2300 | IF 1:500 |
| Puromycin | Mouse | Kerafast | EQ0001 | PLA 1:2,500 |
| Secondary antibodies | ||||
|---|---|---|---|---|
| Antibody | Conjugation | Supplier | Cat # | Dilution |
| anti‐rabbit | HRP | Jackson IR | 711‐035‐152 | WB 1:5,000 |
| anti‐mouse | HRP | Jackson IR | 711‐035‐152 | WB 1:10,000 |
| anti‐rabbit | Alexa Fluor 350 | Thermo Fisher | A11046 | IF 1:800 |
| anti‐rabbit | Alexa Fluor 568 | Thermo Fisher | A11011 | IF 1:800 |
| anti‐rabbit | Alexa Fluor 647 | Thermo Fisher | A21244 | IF 1:800 |
| anti‐Guinea Pig | Alexa Fluor 568 | Thermo Fisher | A11075 | IF 1:800 |
| anti‐mouse | Alexa Fluor 488 | Thermo Fisher | A11001 | IF 1:800 |
| anti‐mouse | Alexa Fluor 568 | Thermo Fisher | A11031 | IF 1:800 |
| anti‐mouse | Alexa Fluor 647 | Thermo Fisher | A21236 | IF 1:800 |
| anti‐rat | Alexa Fluor 568 | Thermo Fisher | A11077 | IF 1:200 |
| anti‐mouse | Alexa Fluor 647 | Thermo Fisher | A21236 | IF 1:800 |
| DNA constructs | |||
|---|---|---|---|
| Backbone | Promoter | Insert | Source |
| pcDNA3.1 | CMV | Homer1c‐GFP | S. Okabe (Tokyo University) |
| pcDNA3.1 | CMV | Homer1c‐DsRed | Mondin et al (2011) |
| pcDNA3.1 | CMV | Homer1c‐BFP | Mondin et al (2011) |
| pcDNA3.1 | CMV | SEP‐GluA2‐3'UTR‐WT/‐MUT | This study |
| pGW1 | CMV | HA‐GluA1 | Gift from D. Choquet (IINS, Bordeaux) |
| pGW1 | CMV | HA‐GluA2 | Gift from D. Choquet (IINS, Bordeaux) |
| pEGFP | CMV | RFP‐SP | Gift from A. Triller (ENS, PARIS) |
| pEGFP | CMV | RFP‐SP‐3'UTR‐WT/‐MUT | This study |
| pEGFP | CMV | shRNA resistant RFP‐SP‐3'UTR‐WT/‐MUT | This study |
| pIRES‐EGFP | CMV | EGFP‐synaptophysin:IRES:TetTx | Gift from D. Choquet/D. Perrais (IINS, Bordeaux) |
| pCAG‐miR30 | CAG | SP‐shRNA with GFP reporter | This study |
| pCAG‐miR30 | CAG | SP‐shRNA with BFP reporter | This study |
| pCAG‐miR30 | CAG | Scrambled shRNA with GFP reporter | Gift from D. Choquet (IINS, Bordeaux) |
| phRL‐CMV | CMV | Luciferase | Favereaux et al (2011) |
| pcDNA3.1 | U6 | miR‐124 | This study |
| Other products | ||
|---|---|---|
| Product | Reference | Supplier |
| miRCURY LNA miRNA Target Site Blocker | 339199 | Qiagen |
| miRCURY LNA miRNA Detection Probe | 339111 | Qiagen |
| TSATM‐Plus Fluorescein System | NEL741001KT | PerkinElmer |
| Duolink | DUO92008 | Merck |
| DAPI Fluoromount‐G | 0100‐20 | SouthernBiotech |
| Bicuculline | 0130 | Tocris |
| TTX | 1069/1 | Tocris |
| NASPM | 2766/10 | Tocris |