Skip to main content
. 2022 Aug 25;41(20):e110486. doi: 10.15252/embj.2021110486

Figure EV3. Expression of CFP‐labeled mitochondria in Thy1‐mito‐CFP Tg (Thy1‐Mito) mouse before and after motor nerve injury.

Figure EV3

  1. Immunostaining of hypoglossal nucleus (XII) of Thy1‐Mito mouse using ATF3 (red) and GFP (green) antibodies at 5 days following hypoglossal nerve injury. XII, hypoglossal nucleus; cc, central canal.
  2. The localization of AnkG and CFP‐labeled mitochondria in the AIS of uninjured and injured hypoglossal motor neurons of Thy1‐Mito mouse.
  3. Immunostaining of the lumbar spinal motor neurons of Thy1‐Mito mouse using ATF3 (red) and GFP (green) antibodies at 5 days following sciatic nerve injury.
  4. The localization of AnkG and CFP‐labeled mitochondria in the AIS of uninjured and injured spinal motor neurons of Thy1‐Mito mouse.
  5. Whole‐mount observation of CFP‐labeled mitochondria (mtCFP) in sciatic nerve.

Data information: Dashed lines outline hypoglossal nucleus (A), spinal cord (C) and axon (E). Closed arrowheads indicate the AIS, while open arrowheads indicate the region where the AIS is disrupted in (B and D). Scale bars, 50 μm (A and C) and 5 μm (B, D and E).