Table 3. In Vitro Enzymatic Activity of 4-Substituted Pyrimidine Analogues on PfGSK3 and PfPK6.

^aIC₅₀ values were determined using the KinaseSeeker assay with 5-fold dilutions, presented as mean values of two experiments performed in duplicate.

^bND: not determined; compound did not reach the threshold of inhibiting ≥70% kinase activity at 1 μM.