Table 5. In Vitro Enzymatic Activity of Hybrid Analogues on PfGSK3 and PfPK6 with Their Associated Ligand Efficiency Values.

^aIC₅₀ values were determined using the KinaseSeeker assay with 5-fold dilutions, presented as mean values of two experiments performed in duplicate.

^bLigand efficiency was calculated as follows: PfPK6 pIC₅₀/number of heavy atoms in the molecule.

^cND: not determined; compound did not reach the threshold of inhibiting ≥70% kinase activity at 1 μM.