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. 2022 Sep 16;65(19):12860–12882. doi: 10.1021/acs.jmedchem.2c00697

Figure 7.

Figure 7

Uptake of lentivirus pseudotyped with the SARS-CoV-2 spike glycoprotein is inhibited by PIKfyve inhibitors. Calu-3 cells were incubated with 1 μM of compound (or DMSO) for 1 h followed by the addition of the lentivirus pseudotyped with the SARS-CoV-2 spike glycoprotein for 12 h. Cells were fixed and stained with Hoechst to label the nuclei. Cells were also imaged for spike expression using spike antibody and visualized via Alexa Fluor 488, following transduction of the pseudovirus. Scale bars (shown in white in the bottom right corner of the images) = 100 μm. Bar graph shows the quantification of SARS-CoV-2 pseudovirus infection from experiments: n = 9 from three independent experiments, mean ± standard deviation (SD); one-way ANOVA followed Tukey’s test. ****p < 0.0001.