Table 5. PIKfyve NanoBRET versus Viral Replication Data.
| compound | PIKfyve NB IC50 (nM) | PIKfyve NB pIC50 | MHV replication IC50 (nM) | MHV replication pIC50 | SARS-CoV-2 replication IC50 (nM) |
|---|---|---|---|---|---|
| 2 | 2540 | 5.61 | 8470 | 5.07 | NTa |
| 5 | 885 | 6.05 | 485 | 6.31 | NT |
| 7 | 380 | 6.42 | 713 | 6.15 | NT |
| 8 | 11.4 | 7.94 | 71.0 | 7.15 | 73.3 |
| 10 | 1170 | 5.93 | 1920 | 5.72 | NT |
| 12 | 4970 | 5.30 | 5350 | 5.27 | NT |
| 13 | 1560 | 5.81 | 666 | 6.18 | NT |
| 14 | >10 000 | 5.00 | 8050 | 5.09 | NT |
| 17 | 4.01 | 8.40 | 23.5 | 7.63 | 19.5 |
| 26 | 12.7 | 7.90 | 74.0 | 7.13 | 23.9 |
| 28 | 700 | 6.15 | 1800 | 5.75 | NT |
| 30 | >10 000 | 5.00 | >10 000 | 5.00 | >10 000 |
| apilimod | 0.312 | 9.51 | 15.7 | 7.80 | NT |
| YM201636 | 73.5 | 7.13 | 250 | 6.60 | NT |
| APY0201 | 1.19 | 8.92 | 40.4 | 7.39 | NT |
| remdesivir | NT | NT | NT | NT | 86.4 |
a
NT: not tested.