Table 1. MSP sequences, hybridization temperatures, and expected amplicon sizes.

Primer name	Base sequence 5′-3′	Base number	Temp. (°C)	Amplicon size (bp)
IL-6 MSP-F	ATAGGTAAGATATTAGGTGAATCGA	25	56	166
IL-6 MSP-R	TTTCTAAAACTATTATAAAAATAAAACGTA	30	53	166
IL-6 USP-F	ATAGGTAAGATATTAGGTGAATTGA	25	55	166
IL-6 USP-R	TTTCTAAAACTATTATAAAAATAAAACATA	30	52	166
HIF-3A MSP-F	TAGGTTTGGCGTGGTATAGTTAATC	25	60	259
HIF-3A MSP-R	CCCGAAACGTTCTTAACTCG	20	57	259
HIF-3A USP-F	TTTAGGTTTGGTGTGGTATAGTTAATTG	28	59	262
HIF-3A USP-R	CCCCAAAACATTCTTAACTCAC	22	57	262

HIF-3A: hypoxia factor 3A; MSP: methylation-specific primer; USP: unmethylated-specific primer; F: forward; R: reverse; IL-6: interleukin-6. Primers designed with the “MethPrimer” database http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi.