FIGURE 4.

DMSCs‐derived EDIL3 activated FAK/MEK/ERK signal pathway in HUVECs. (A) The images of protein bands of β‐actin, FAK/p‐FAK, MEK/p‐MEK, ERK1/2 and p‐ERK1/2. The molecular weights were, respectively, 49 kDa, 116 kDa, 61 kDa, 50 kDa, 45 kDa, 44 kDa and 45 kDa. (B) Quantitative analysis of the protein levels. (C) The molecule model of regulating ECs functions by DMSCs‐derived EDIL3. P‐DMSCs secreted EDIL3, which upregulated integrin αvβ3 expression through RGD‐motif structure in HUVECs. Then, the downstream FAK was activated by integrins and translated into phosphorylated FAK (p‐FAK). The p‐FAK further activated MEK and ERK1/2. The integrin pathway transduced the signal into cell nucleus and regulated genes expression