FIGURE 4.

Silencing SRSF3 expression induces p21 activation accompanied by a decrease in the expression of fibrosis effector genes in CMFs in vitro. (A‐B) Transfection of siSRSF3 in CMFs significantly downregulated the expression of SRSF3 at the mRNA level (A) and protein level (B). (B1) Representative pattern of Western blot for siSRSF3‐ and siNC‐treated group. (B2) Quantitative analysis of (B1). C: RIP‐qPCR revealed that SRSF3 was able to bind with p21 in CMFs. (D‐E) Transfection of siSRSF3 in CMFs upregulated the expression of p21 significantly at the mRNA level (D) and protein level (E) but not p16 and p53. (E1) Representative pattern of Western blot for siSRSF3‐ and siNC‐treated group. (E2) Quantitative analysis of E1. F: SRSF3 silencing in CMFs decreased the expression of fibrosis effector genes (PAI‐1, TSP‐1 and α‐SMA). n = 3