Fig. 5.

Autophagy participates in the degradation of aggregated claudin-5 in the cytosol. A C57BL/6 mice were exposed to HH (7600 m above sea level) for 24 h. Brain slices were colabelled with claudin-5 and LC3 antibodies to observe the colocalization of claudin-5 and LC3 (n = 10). B to E bEnd.3 cells were pretreated with 50 nmol/L Rapa or 10 mmol/L 3-MA for 2 h, followed by hypoxia treatment for 24 h. Cell permeability was determined by TEER (B) and leakage of 40 kD FITC-dextran (C) to analyse the effect of autophagy on endothelial barrier function. Claudin-5 and LC3 antibodies were labelled by immunofluorescence (D) to count the fluorescence intensity of claudin-5 (E) (*P < 0.05, **P < 0.01 and ***P < 0.001)