TABLE 2.
Immune responses against recombinant mycobacterial proteins in field animals that were positive by the single comparative tuberculin testa
| Animal | Skin test thickness (mm)b | In vitro proliferative
responsec
|
||||
|---|---|---|---|---|---|---|
| PPD-Md | PPD-A | ESAT-6e | MPB64 | MPB83 | ||
| 1 | 36 | 54.2 (27.8) | 54.7 (28.0) | 2.6 (1.3) | 38.4 (19.7) | 15.2 (7.8) |
| 2 | 31 | 233.0 (37.5) | 223.0 (30.0) | 260.8 (41.9) | 2.8 (0.5) | 102.0 (16.5) |
| 3 | 30 | 95.6 (38.5) | 85.1 (34.2) | 30.1 (12.1) | 3.6 (1.4) | 21.7 (8.7) |
| 4 | 22 | 161.0 (35.6) | 116.5 (25.8) | 199.0 (44.0) | 1.0 (0.2) | 2.1 (0.5) |
| 5 | 18 | 466.1 (66.7) | 66.5 (9.5) | 351.7 (50.3) | 3.8 (0.5) | 18.2 (2.6) |
| 6 | 14 | 65.0 (14.2) | 7.2 (1.6) | 4.3 (0.9) | 0.8 (0.2) | 1.8 (0.4) |
| 7 | 10 | 23.6 (13.8) | 19.2 (11.2) | 0.5 (0.3) | 1.9 (1.1) | 0.8 (0.4) |
| 8 | 9 | 119.4 (35.4) | 74.2 (20.5) | 29.4 (8.1) | 27.0 (7.5) | 1.6 (0.4) |
| 9 | 8 | 116.9 (35.4) | 198.1 (60.0) | 2.8 (0.8) | 2.3 (0.7) | 1.5 (0.5) |
| 10 | 8 | 25.1 (8.6) | 18.3 (6.3) | 8.8 (3.0) | 1.4 (0.5) | 8.2 (2.8) |
| 11 | 7 | 92.9 (45.8) | 64.5 (31.8) | 7.7 (3.8) | 11.5 (5.6) | 5.9 (2.9) |
| 12 | 7 | 233.0 (37.5) | 223.0 (36.0) | 260.8 (41.9) | 2.8 (0.5) | 102.0 (16.5) |
| 13 | 7 | 68.7 (14.3) | 34.0 (7.1) | 3.4 (0.7) | 1.9 (0.4) | 2.5 (0.5) |
| 14 | 6 | 25.5 (30.4) | 9.7 (11.5) | 12.6 (15.0) | 4.7 (5.6) | 13.4 (16.0) |
| 15 | 6 | 219.1 (28.7) | 18.5 (2.4) | 67.7 (8.9) | 0.8 (0.1) | 18.6 (2.4) |
| 16 | 5 | 3.5 (0.1) | 8.0 (0.3) | 2.1 (0.1) | 1.3 (0.1) | 1.5 (0.1) |
| 17 | 5 | 57.1 (14.6) | 8.1 (2.1) | 3.2 (0.8) | 4.6 (1.2) | 0.9 (0.2) |
| 18 | 3 | 377.0 (56.6) | 321.0 (48.2) | 55.7 (8.2) | 0.9 (0.1) | 1.0 (0.2) |
PBMC from field reactors were isolated and cultured within 8 h of blood sampling. Antigen concentrations were 10 μg/ml for PPD-M, PPD-A, MPB83, and MPB64 and 5 μg/ml for ESAT-5.
Skin test measurements at 72 h are thickness induced with PPD-A substracted from thickness induced with PPD-M.
Results are expressed as SIs (defined as radioactivity with antigen/radioactivity without antigen), with results for the individual antigen (in kilocounts per minute) shown in parentheses.
If the SI for PPD-M was greater than that for PPD-A, the result was deemed positive; positive responses are underlined (responder frequency was 83% (15 of 18).
For recombinant proteins and cocktails, if the SI was ≥3 and the radioactivity with antigen was ≥1,000 cpm, a response was deemed positive; positive responses are in bold. Responder frequencies were as follows: ESAT-6, 66% (12 of 18); MPB64, 33% (6 of 18); and MPB83, 50% (9 of 18).