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. 2022 Oct 3;13:993788. doi: 10.3389/fimmu.2022.993788

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Copyright © 2022 Huang, Yan, Dong, Jiao, Wang, Li and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miRNA-887-5p increased in peritoneal macrophages of endometriosis. (A) Dual-luciferase reporter assay was conducted in 293T cells co-transfected with miR-NC or miR-887-5p. (B) Vector construction for luciferase experiment. (C) MST1(STK4)-miR-887-5p UTR sequencing peak. (D) The mRNA levels of miR-887-5p in human peritoneal macrophages with or without endometriosis by RT-qPCR. (E, F) Representative western blot of protein expression after transfection. (G) The mRNA level of MST1 and p38-MAPK after miR-887-mimics and -inhibitor transfection. (H) The cell viability of THP-1 after transfection. *P < 0.05, **P < 0.01, ***P < 0.001 (Control group peritoneal macrophages vs. endometriosis group peritoneal macrophages). Values represent the mean ± standard error. ns means not statistically significant.