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. Author manuscript; available in PMC: 2023 Aug 17.
Published in final edited form as: Neuron. 2022 Jun 13;110(16):2664–2679.e8. doi: 10.1016/j.neuron.2022.05.015

Figure 5. DRN5-HT ensembles are specifically excited by the first interaction with a novel mouse.

Figure 5.

A) Diagram of the social interaction paradigm.

B) Fraction of time mice spend interacting. n = 13 mice (minutes 5 and 6, n = 12 and 11, respectively). Mixed-effects analysis, p = 0.0035. Paired t-test between 1 and 2 minutes, p < 0.0001.

C) Population-level response of a representative animal to the first interaction (black) compared all other interactions (purple). Shading represents ±SEM.

D) Example single cell response to first interaction.

E) Relative proportion of cells with a significant response to the first interaction (53% excited, 215/404 cells, shuffle test, α = 0.05, n = 13 mice).

F) Example ensembles. Ensemble 1 is excited by first interaction (p = 0.0008), while ensemble 2 is non-selective (p = 0.8548). Purple shading represents epochs in which mice are in close physical contact.

G) Spatial distribution of cells in (F).