Antibodies
|
anti-GFP rabbit polyclonal Alexa Fluor™-488 conjugate |
Invitrogen |
Cat#A-21311 |
Anti-Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8 |
Millipore Sigma |
Cat#MAB5278 |
Cy5 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) |
Jackson ImmunoResearch Laboratories |
Cat#111-175-144 |
goat anti-rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody AlexaFluor 555 |
Invitrogen |
Cat#A-21428 |
anti-serotonin (5-HT) transporter (602-622) rabbit polyclonal antibody |
Millipore Sigma |
Cat#PC177L-100UL |
Bacterial and Virus Strains
|
AAVDJ-DIO-EF1a-GCaMP6m |
Stanford |
Cat#GVVC-AAV-092 |
AAV9-CAG-flex-GCaMP6m-WPRE-S |
U Penn Vector Core |
Cat#AV-9-PV2817 |
AAV2-retro-DIO-EF1a-GCaMP6m |
Stanford |
N/A, custom preparation |
AAV5-flex-GFP |
UNC Gene Therapies |
Cat#AV4531 |
AAV9-CAG-flex-tdTomato-WPRE |
U Penn Vector Core |
Cat#AV-9-ALL864 |
AAVDJ-hSyn-GCaMP6m |
Stanford |
Cat#GVCC-AAV-095 |
Chemicals, Peptides, and Recombinant Proteins
|
Sucrose |
Sigma-Aldrich |
Cat#S7903-5KG |
Quinine hydrochloride dihydrate |
Sigma-Aldrich |
Cat#Q1125-10G |
Vectashield Antifade Mounting Medium |
Vector Laboratories |
Cat#H-1000 |
Experimental Models: Organisms/Strains
|
C57BL/6J mice |
Jackson Laboratory |
Cat#000664; RRID:SCR_004633; https://www.jax.org/index.html
|
B6.129(Cg)-Slc6a4tm1(cre)Xz/J (SERT-Cre) mice |
Jackson Laboratory |
Cat#014554; https://www.jax.org/index.html
|
Software and Algorithms
|
Ethovision XT11 |
Noldus |
http://www.noldus.com; RRID:SCR_000441 |
Inscopix Data Processing Software |
Inscopix |
https://www.inscopix.com
|
Observer XT |
Noldus |
http://www.noldus.com/human-behavior-research/products/the-observer-xt
|
MATLAB |
Mathworks |
https://www.mathworks.com/products/matlab/; RRID:SCR_001622 |
CNMF-E |
GitHub |
https://github.com/zhoupc/CNMFE
|
Image J |
Fiji |
https://imagej.nih.gov/ij/
|
CalTracer 3 (beta) |
Yuste Lab |
http://www.columbia.edu/cu/biology/faculty/yuste/methods
|