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. Author manuscript; available in PMC: 2023 Aug 17.
Published in final edited form as: Neuron. 2022 Jun 13;110(16):2664–2679.e8. doi: 10.1016/j.neuron.2022.05.015
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
anti-GFP rabbit polyclonal Alexa Fluor™-488 conjugate Invitrogen Cat#A-21311
Anti-Tryptophan Hydroxylase/Tyrosine Hydroxylase/Phenylalanine Hydroxylase Antibody, clone PH8 Millipore Sigma Cat#MAB5278
Cy5 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) Jackson ImmunoResearch Laboratories Cat#111-175-144
goat anti-rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody AlexaFluor 555 Invitrogen Cat#A-21428
anti-serotonin (5-HT) transporter (602-622) rabbit polyclonal antibody Millipore Sigma Cat#PC177L-100UL
Bacterial and Virus Strains
AAVDJ-DIO-EF1a-GCaMP6m Stanford Cat#GVVC-AAV-092
AAV9-CAG-flex-GCaMP6m-WPRE-S U Penn Vector Core Cat#AV-9-PV2817
AAV2-retro-DIO-EF1a-GCaMP6m Stanford N/A, custom preparation
AAV5-flex-GFP UNC Gene Therapies Cat#AV4531
AAV9-CAG-flex-tdTomato-WPRE U Penn Vector Core Cat#AV-9-ALL864
AAVDJ-hSyn-GCaMP6m Stanford Cat#GVCC-AAV-095
Chemicals, Peptides, and Recombinant Proteins
Sucrose Sigma-Aldrich Cat#S7903-5KG
Quinine hydrochloride dihydrate Sigma-Aldrich Cat#Q1125-10G
Vectashield Antifade Mounting Medium Vector Laboratories Cat#H-1000
Experimental Models: Organisms/Strains
C57BL/6J mice Jackson Laboratory Cat#000664; RRID:SCR_004633; https://www.jax.org/index.html
B6.129(Cg)-Slc6a4tm1(cre)Xz/J (SERT-Cre) mice Jackson Laboratory Cat#014554; https://www.jax.org/index.html
Software and Algorithms
Ethovision XT11 Noldus http://www.noldus.com; RRID:SCR_000441
Inscopix Data Processing Software Inscopix https://www.inscopix.com
Observer XT Noldus http://www.noldus.com/human-behavior-research/products/the-observer-xt
MATLAB Mathworks https://www.mathworks.com/products/matlab/; RRID:SCR_001622
CNMF-E GitHub https://github.com/zhoupc/CNMFE
Image J Fiji https://imagej.nih.gov/ij/
CalTracer 3 (beta) Yuste Lab http://www.columbia.edu/cu/biology/faculty/yuste/methods