Table 1. Summed FluoroSpot IFN-γ, IL2, and IFN-γ+IL2 responses to S, N and M proteins.
| Group | # | IFN-γ | IL2 | IFN-γ+IL2 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| S | N | M | S | N | M | S | N | M | ||||
| 1 | Uninfected & unvaccinated | 12 | Median Q1-Q3 | 48 35–121 | 1 0–18 | 22 11–41 | 42 25–76 | 10 4–29 | 3 0–5 | 0 0–5 | 0 0–0 | 0 0–0 |
| # (%) Pos | 8 (67) | 1 (8) | 5 (42) | 1 (8) | 1 (8) | 0 (0) | 1 (8) | 0 (0) | 0 (0) | |||
| 2 | Uninfected &vaccinated | 179 | Median Q1-Q3 | 195* 133–308 | 20* 8–45 | 25 14–53 | 246* 148–393 | 33* 17–57 | 3 0–10 | 40* 18–73 | 3* 0–10 | 0 0–0 |
| # (%) Pos | 159 (89) | 49 (27) | 93 (52) | 166 (93) | 65 (36) | 1 (0.6) | 49 (27) | 5 (3) | 0 (0) | |||
| 3 | Infected & vaccinated | 8 | Median Q1-Q3 | 578^ 391–808 | 189^ 80–230 | 168^ 86–250 | 490^ 344–550 | 130^ 96–252 | 171^ 54–268 | 110^ 113–155 | 34^ 21–69 | 54^ 28–74 |
| # (%) Pos | 8 (100) | 7 (88) | 8 (100) | 8 (100) | 6 (75) | 7 (88) | 7 (88) | 4 (50) | 5 (63) | |||
The sum of magnitudes of IFN-γ, IL2 and IFN-γ+IL2 responses to S, N and M individual peptide subpools are expressed as the median, and first and third quartiles (Q1 and Q3, respectively) responses (spot forming cells/million PBMC, sfc/m), and frequencies are expressed as the number (#) and % of the participants that were positive (as defined in Methods).
Initial omnibus null hypotheses comparing the summed median magnitude (Kruskal-Wallis) to all pools of each protein were interpreted using an alpha = 0.05. When rejected, pairwise comparisons were performed and interpreted using a Bonferroni-adjusted alpha = 0.0167. *Significant differences medians in Group 2 compared to Group 1
^Significant differences in medians in Group 3 compared to Group 2. Analysis of the frequency of response (Fisher’s Exact) not done.