Table 3. FluoroSpot IL2 responses to individual S, N, and M peptide subpools.

	IL2
	GROUP 1 N = 12		GROUP 2 N = 179		GROUP 3 N = 8		KW	Chi-Sq
Peptide Pool	Median Q1-Q3	# Pos (%)	Median Q1-Q3	# Pos (%)	Median Q1-Q3	# Pos (%)	P Mag	P Fre
Sp1	3 0–8	0 (0)	17* 7–30	59 (33)*	24 15–34	4 (50)	**
Sp2	0 0–3	0 (0)	34* 23–66	123 (69)*	76 43–116	7 (88)	**	**
Sp3	0 0–5	0 (0)	22* 8–43	77 (43)*	29 23–48	4 (50)	**
Sp4	0 0–3	0 (0)	22* 0–235	85 (47)*	41 29–68	6 (75)	**	**
Sp5	4 0–9	0 (0)	8 10–40	35 (20)	41^ 19–48	5 (63)^	**	**
Sp6	0 0–0	0 (0)	3* 0–10	9 (5)	6 0–18	1 (13)
Sp7	2 0–13	0 (0)	48* 28–82	145 (81)*	70^ 64–168	8 (100)	**	**
Sp8	3 0–8	0 (0)	38* 19–60	120 (67)*	59 40–84	8 (100)	**	**
Sp9	6 2–11	0 (0)	23* 15–42	84 (47)*	49^ 40–70	8 (100)^	**	**
Sp10	6 3–9	1 (8)	6 0–14	19 (11)	19 6–33	2 (25)
Np1	0 0–3	0 (0)	5* 0–10	13 (7)	25^ 18–66	3 (38)	**	**
Np2	3 0–6	1 (8)	5 0–10	10 (6)	18^ 9–28	2 (25)	**
Np3	0 0–4	0 (0)	3 0–10	13 (7)	25^ 4–41	3 (38)	**	**
Np4	8 1–16	0 (0)	16 6–30	57 (32)*	83^ 35–116	6 (75)	**	**
Mp1	0 0–3	0 (0)	0 0–4	1 (0.6)	21^ 13–29	3 (38)^	**	**
Mp2	0 0–4	0 (0)	2 0–6	0 (0)	130^ 44–243	7 (88)^	**	**

IL2 responses to individual peptide subpools for the S, N and P proteins were expressed as median and range of responses (spot forming cells/million PBMC, sfc/m), and number and % participants that were positive.

Initial omnibus null hypotheses comparing the magnitude (Kruskal-Wallis) and frequency (Fisher’s Exact) of responses of all groups to each of the subpools were interpreted using an alpha = 0.05. When rejected, pairwise comparisons were performed and interpreted using a Bonferroni-adjusted alpha = 0.0167.

*Significant differences between Group 2 compared to Group 1

^significant differences between Group 3 compared to Group 2.

In addition, the magnitudes, and frequencies of responses of each Group were also compared by Kruskall-Wallis (KW) or Chi-Square (Chi-Sq).

**Significant p values indicate that there were significant differences between each group.