Figure 4. Validation with polyacrylamide beads.

(a) Deformation of PAAm beads with different acralymide-bisacrylamide total monomer concentrations (soft 3.9%, medium 5.9%, stiff 6.9%) at different positions in the channel. (b) AFM data (G' and G" versus frequency, mean values from 14 stiff PAAm beads (blue/orange circles), solid lines are the fit of Equation 2 to the data). G’ (c) and G” (d) for stiff beads at 2 bar. White squares indicate binned median values, blue and orange solid lines are the fit of Equation 2 to the data. (e) AFM-measured stiffness compared to the stiffness versus pressure measured with shear flow deformation cytometry (SF-DC) for differently stiff PAAm beads. (f) AFM-measured fluidity compared to fluidity versus strain measured with SF-DC for the same beads as in e.

Figure 4—figure supplement 1. Frequency-dependent shear modulus $G^{\prime }$ (blue) and loss modulus $G^{\prime \prime }$ (orange) of polyacrylamide (PAAm) beads (soft: 3.9% $C_{\mathrm{AAmBis}}$ (top row); medium: 5.9% $C_{\mathrm{AAmBis}}$ (middle row); stiff: 6.9% $C_{\mathrm{AAmBis}}$ (bottom row)) measured with atomic force microscopy (left column) and shear flow deformation cytometry (middle and right columns).

Lines show the fit of Equation 2 to the AFM data (left column, solid circles show mean values from $n\ge 9$ beads), or to the shear flow cytometry data (middle and right column, each dot represents the data from one bead, colors represent Gaussian kernel density, white squares show the median values over equal-sized bins).