Figure 6. Dose response measurements.

a, Dose response curve of NIH-3T3 cells treated with different concentrations of latrunculin B. Stiffness is normalized to the stiffness of DMSO-treated cells, grey points indicate n=4 independent measurements for each concentration, each measurement is the average of a 0.5, 1, 2, and 3 bar measurement, boxplot indicate median (orange line) and 25 and 75 percentiles, whiskers indicate 5 and 95 percentiles. Blue line is the fit of the Hill-Langmuir-equation to the data, with an EC50 of 31.2 nM. b, Dose-response curve of HL 60 cells. Hill-Langmuir fit gives an EC50 value of 25.9 nM.