Figure 7.

JNK inhibitor SP600125 and P38 inhibitor SB20358 reversed the anti-tumor effect induced by NTF3. A, B CCK8 and colony formation experiments suggested that the addition of inhibitors reversed the inhibitory effect of NTF3-induced proliferation. C, D Wound-healing, invasion, and migration experiments suggested that the addition of inhibitors reversed the invasion and migration effects of NTF3. E Set of 4 groups in HCCLM3: 1) Vector, 2) NTF3 group, 3) NTF3+SP600125, 4) NTF3+SB20358; the changes in apoptosis were detected by flow cytometry. F WB detected the changes in the total and phosphorylated JNK and P38 levels. G WB detected the changes in the apoptosis-related proteins in the 4 groups. *P < 0.05; **P < 0.01; ***P < 0.001.