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. 2022 Sep 11;18(15):5724–5739. doi: 10.7150/ijbs.76140

Figure 3.

Figure 3

HDAC5 knockdown inhibits TGF-β1-induced HSF activation. (A) Identification of shHDAC5 efficiency in HSFs. (B) EdU (green) proliferation assay for cultured HSFs after incubation with TGF-β1 for 24 h. (Scale bar = 100 µm). (C) Images and quantification of immunofluorescence staining for α-SMA in different groups. α-SMA is labeled in green. (Scale bar = 50 µm). (D) Images and quantification of wound healing assays in different groups 12 h after the addition of TGF-β1. (E) Images and quantification of collagen gel contraction assays in different groups on Day 3 after TGF-β1 addition. Dashed lines indicate the areas of collagen gel. (F) The protein levels of collagen I and III in HSFs pretreated with TGF-β1 for 24 h. Data are presented as the means with SEs (n = 3 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001, NS = not significant.