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. 2022 Sep 11;18(15):5724–5739. doi: 10.7150/ijbs.76140

Figure 6.

Figure 6

HDAC5 interacts with MEF2A and diminishes its transcriptional activity on the Smad7 promoter region. (A) Co-IP assay between HDAC5 and MEF2A in HSFs. (B) ChIP assay confirmation of the binding of MEF2A to the Smad7 promoter region in the shCtrl and shHDAC5 groups of HSFs. DNA immunoprecipitated by MEF2A antibody or immunoglobulin G (IgG CTL) was amplified by RT-qPCR using primers for the Smad7 promoter. (C) Activation of the Smad7 promoter luciferase reporter by MEF2 and attenuation by HDAC5 in HSFs. (D) Prediction of MEF2A-binding sites in the Smad7 promoter region using JASPAR software. (E) Effects of MEF2A-binding site mutations in the Smad7 promoter on transcriptional activation by MEF2A. Data are presented as the means with SEs (n = 3 independent experiments). ***P < 0.001.