Figure 3.
3D-tumor slice culture increases the number of live cells and maintains the immune components of their original tumors. This figure was modified from our previous publication 27 and gained approval from the correspondence authorship. A. Observation of tumor growth in 3D-TSCs derived from primary tumors formed by B477-GFP cells injected into the mammary fat pad of nude mice. Scale bar: 1 mm. B. Time-lapse of cell viability in 3D-TSCs derived from B477-GFP mouse tumors. C. Immunofluorescence staining of immune biomarkers derived from genetically engineered Brca1Co/Co; MMTV-Cre mouse in immunocompetent hosts. Blue is nuclear counterstaining by hematoxylin, and brown staining is positive protein staining by DAB. CD3e/CD8a: T lymphocytes; CD11b, macrophages and microglia marker; CD45, T, NK, dendritic, and lymphokine-activated killer cells marker; Scale bar: 100 µm. D. Pearson correlation coefficient identifying the correlation of gene expression between Day 1 to Day 8 with Day 0 in T cells and B cells.