Fig. 4.

Effects of dietary porcine bile acids supplementation on KEGG pathway and orthologous genes among groups, predicted by Tax4fun. BA = bile acids. (A) Changes in KEGG pathways associated with lipid metabolism. The color of bubbles represents the P-value of student's t test between bile acids treatment and the control. The size of bubbles represents the -log₂ value of the functional proportion of the pathway. X-axis represents log₂ value of fold change to the control group. (B) Changes of genes related to bile acid metabolism enzymes. K00038 is the KEGG number of the 3 alpha-hydroxysteroid dehydrogenase; K00076 is the KEGG number of the 7 alpha-hydroxysteroid dehydrogenase; K01442 is the KEGG number of the choloylglycine hydrolase. (C) Changes of genes related to acetic acid metabolism enzymes. K00625 is the KEGG number of phosphate acetyltransferase; K00925 is the KEGG number of acetate kinase. (D) Changes of genes related to propionic acid metabolism enzymes. K01026 is the KEGG number of the propionate CoA-transferase; K14469 is the KEGG number of the acrylyl-CoA reductase (NADPH); K15020 is the KEGG number of the acryloyl-CoA reductase. (E) Changes of genes related to butyric acid metabolism enzymes. K00929 is the KEGG number of the butyrate kinase; K01034 is the KEGG number of the acetate CoA-transferase alpha subunit; K01035 is the KEGG number of the acetate CoA-transferase beta subunit. ∗, 0.01 < P < 0.05; ∗∗, P < 0.01.