Table 2.
Primers for quantitative real-time reaction (qPCR).
| Gene Name | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| Rat primers | ||
| GAPDH | AGCCCAGAACATCATCCCTG | CACCACCTTCTTGATGTCATC |
| Sox-9 | AGAGCGTTGCTCGGAACTGT | TCCTGGACCGAAACTGGTAAA |
| Col 2a1 | AACCCAAAGGACCCAAATAC | CCGGACTGTGAGGTTAGGAT |
| ACAN | TTGTGACTCTGCGGGTCATC | GTCCCTAGGAGGGCCTTCAG |
| MMP13 | AGGCCTTCAGAAAAGCCTTC | GAGCTGCTTGTCCAGGTTTC |
| Col 10a1 | ATATCCTGGGGATCCAGGTC | TCCAGGTTCACCTCTTGGAC |
| Runx2 | GAACCAAGAAGGCACAGAC | AATGCGCCCTAAATCACTG |
| Human primers | ||
| Sox-9 | TGGGCAAGCTCTGGAGACTTC | ATCCGGGTGGTCCTTCTTGTG |
| Runx2 | GACAAGCACAAGTAAATCATTGAACTACAG | GTAAGGCTGGTTGGTTAAGAATCTCTG |
GAPDH: glyceraldehyde-3-phosphate dehydrogenase, Sox-9: SRY (Sex-Determining Region Y)-box transcription factor 9, Col 2a1: Collagen type II alpha 1 chain, ACAN: Aggrecan, MMP13: Matrix metallopeptidase 13, Col 10a1: Collagen type X alpha 1 chain, Runx2: Runt-related transcription factor 2.