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. 2022 Mar 22;29(10):1355–1372. doi: 10.1038/s41417-022-00451-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Immortalized cells infected with pRetroX-TetOne puro empty vector (empty) or pRetroX-TetOne puro-ALKBH5 (ALKBH5) were used to assess the function of ALKBH5-overexpressed cells. The concentration of doxycycline (DOX) was 100 ng/mL. A Western blot analysis demonstrated ALKBH5 protein levels in ALKBH5-overexpressed HEK293 and BEAS2B cells. B, C Cells infected with pRetroX-TetOne puro-ALKBH5 with DOX were designated as ALKBH5 overexpression (OE), whereas those without DOX were designated as negative control (NC). Cell proliferation relative to baseline in ALKBH5 OE HEK293 and BEAS2B cells were assessed using the CCK-8 assay (n = 3). D–G The migration ability of HEK293 and BEAS2B cells was assessed via wound-healing assay. Representative images of the wound-healing assay for HEK293 (D) and BEAS2B (F) cells. Wound areas relative to baseline at each time point were compared between ALKBH5 OE and NC HEK293 (E) and BEAS2B (G) cells (n = 3). Results are presented as mean ± SD. *P < 0.05 indicates a significant difference between the indicated groups.