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Western blot of affinity-purified H. hepaticus recombinant protein (GST-MAP18) and control protein (GST) probed with pooled sera from mice infected with H. hepaticus, H. muridarum, or H. bilis or with pooled sera from uninfected mice. Note that antibodies to GST-MAP18 are detected only for mice infected with H. hepaticus. Each lane contains 10 μg of protein. M, prestained low-range SDS-polyacrylamide gel electrophoresis protein standards (Bio-Rad Laboratories).
