Figure 3.

X probe for highly specific recognition of nucleic acids. A) Strands m and f hybridize to both the MB probe and the targeted analyte to form a fluorescent complex stabilized by a DNA four-way junction structure ⁶⁰. Right panel: melting profiles of the fluorescent complexes in the presence of fully matched and mismatch analyte. Dash-dotted line - the MB probe alone; Dashed line - the binary probe without analyte present ⁶¹. B) Binary ASO consists of biASOa and biASOb strands that form a 4WJ complex with a targeted RNA only in the presence of a biomarker strand. Right panel: RNase H-dependent cleavage of the targeted RNA by biASO in comparison with the traditional ASO ²⁶.