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. 2022 Sep 25;12(16):6883–6897. doi: 10.7150/thno.77457

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Screening of Near-Infrared Afterglow Nano-Reporter. (A) Molecular structures of various afterglow molecules and the illustration of the preparation of SPN via nanoprecipitation. (B) Transmission electron microscope image of SPN(NIR-3). (C) Absorption spectra of various SPN. (D) Fluorescence spectra of various SPN. (E) Afterglow intensities of SPN(NIR-1/NIR-2/NIR-3) (The inset is the afterglow image). (F) Afterglow emission of various SPN, obtained through different channels (GFP: 510-570 nm; DsRed: 570-650 nm; Cy5.5: 690-770 nm; ICG: 820-880 nm). (G) The corresponding afterglow intensity, quantified from (F).