Skip to main content
. 2022 Sep 25;12(16):6826–6847. doi: 10.7150/thno.72685

Figure 1.

Figure 1

Characterization of brain endothelial-like cells (iBECs) from APOE3 and APOE4-carrying human iPSCs. (A) Representative immunofluorescence images of Occludin (green), Claudin-5 (magenta) and ZO-1 (green) in APOE3 and APOE4 iPSC-derived iBECs (scale bar = 100 µm, nuclei stained using Hoechst). (B) Trans-endothelial electrical resistance (TEER, Ohm x cm2) and passive 5 kDa dextran permeability (24 h clearance volume) in APOE3 and APOE4 iBECs (separate cell lines shown as different symbols). (C) Relative gene expression of blood-brain barrier (BBB) and endothelial cell markers in APOE3 iPSCs vs iBECs (top row) and APOE4 iPSCs vs iBECs (bottom row). (D) Relative gene expression of BBB and endothelial cell markers in APOE3 and APOE4 iBECs (separate cell lines shown as different symbols). N = 3 biological replicates and a minimum of n = 3 independent replicates per line. * P < 0.05, ** P < 0.01, **** P < 0.0001 by unpaired t-test with Welch's correction, error bars = SEM.