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. 2022 Sep 25;12(16):6826–6847. doi: 10.7150/thno.72685

Figure 4.

Figure 4

Effect of FUS+MB on APOE3 and APOE4 iAstrocytes (A) Representative immunofluorescence images of APOE3 and APOE4 iAstrocytes in untreated (UT) and focused ultrasound + microbubble (FUS+MB) conditions 1 h and 24 h following treatment stained with AQP4 (red) and GFAP (cyan; Hoechst counterstain, scale bar = 100 µm). (B) Lactate dehydrogenase (LDH) release (shown as absorbance, AU) in UT and FUS+MB treated APOE3 and APOE4 iAstrocytes 1 h and 24 h following treatment. (C) Relative gene expression of astrocyte markers AQP4 and GFAP in UT and FUS+MB treated APOE3 and APOE4 iAstrocytes 1 h and 24 h after treatment. (D) Relative gene expression of inflammatory markers IL-1β, IL-8 and IL-6 in UT and FUS+MB treated APOE3 and APOE4 iAstrocytes 1 h and 24 h after treatment. N = 2 biological replicates and minimum n = 3 independent replicates per line. * P < 0.05, ** P < 0.01 by unpaired t-test with Welch's correction or by one-sample t-test (fold change values), error bars = SEM.