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. 2022 Oct 3;12(16):6915–6930. doi: 10.7150/thno.78171

Figure 1.

Figure 1

Generation of human/mouse chimeric anti-CDCP1 antibody ch10D7 from mouse monoclonal antibody 10D7. A. Schematic illustrating the generation of ch10D7 from murine 10D7. B. Comparative analysis of ch10D7 and 10D7 binding affinity to CDCP1-ECD by SPR. Top: SPR-derived sensograms of ch10D7 and 10D7 binding to various concentrations of recombinant CDCP1-ECD. Bottom: Table summarizing association (Ka), dissociation (Kd) and affinity (KD) constants of ch10D7 and 10D7 to CDCP1-ECD. C. Comparison of binding of ch10D7 and 10D7, fluorescently labelled with the dye Atto-550, to PDAC (TKCC05) and ovarian cancer (HEY) cells by flow cytometry. Top panel: Competition experiments in which cells were incubated with either one labelled antibody or the combination of one labelled antibody with the other unlabelled antibody (ratio 1:1). Bottom panel Saturation experiments in which CDCP1 binding sites for one labelled antibody were blocked with saturating amounts of the other unlabelled antibody (ratio 1:10). CDR, Complementarity-determining region; -L, Light; -H, Heavy; VL, Variable light; VH, Variable heavy; CH, Constant heavy; CL, Constant light.