Skip to main content
. 2022 Oct 3;12(16):7051–7066. doi: 10.7150/thno.73099

Figure 3.

Figure 3

ERK inactivation promotes EMT in NSCLC cells. (A) RNA-seq and GSEA showed enrichment of EMT related genes among BVD-treated A549 cells. (B-C) Effect of ERKi treatment on expression of EMT markers. A549 and HCC827 cells were treated with BVD or SCH at 2.5 µM for 5 days. Immunofluorescence staining (B) and immunoblotting (C) were conducted for determining E-Cad and Vimentin expression. The pRSK level was examined to show inhibition of the ERK activity by BVD and SCH in (C). (D-E) Effects of ERKi treatment on cell migration of NSCLC cell lines. A549 (D) and HCC827 (E) cells were treated with BVD or SCH at 2.5 µM for 5 days. The migration ability of these cells was assessed using the wound healing assay. (F-G) Effects of ERKi treatment on cell invasion of NSCLC cells. A549 (F) and HCC827 (G) cells were treated with BVD or SCH at 2.5 µM for 5 days. The invasion ability of these cells was assessed using the transwell cell invasion assay. H, Effects of BVD treatment on expression of EMT markers in NSCLC tumor tissues. PDX72-bearing NSG mice were treated with BVD for 10 days, E-Cad and Vimentin expression in tumor tissues was determined using IHC. N = 3, bar: SD, **: P < 0.01.