Figure 1.

Animal/equipment setup for von Frey mechanical testing

(A) Diagram of a von Frey mechanical testing rack. This setup can be built in-house using materials described in the key resources table.

(B) Aerial view diagram of plexiglass mouse enclosures with measurements.

(C) Example image of mice on the von Frey mechanical testing rack. Animals are placed into individual chambers on a mesh surface, allowing experimenters full visibility and access to paws during von Frey testing. Panels A and B were created in Biorender.com.