Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Dissection and preparation of eye cups for RPE isolation
After extraocular tissue is removed from eye (A) and eye is disinfected with PVP prep solution (B), a scalpel is used to make an incision in the eye (C). Clean scissors are used to cut around scleral-corneal interface and anterior chamber (D), vitreous (E), and retina (F) is be removed. Eye cup is then placed in a 6-well plate and filled with L-15 medium (20°C–25°C) (G and H).
