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Panel of tested antibodies stained with suggested antibody dilutions from Table 1
Transwell membranes with primary porcine RPE, aged 20 weeks, were fixed and cryosectioned using the outlined protocol and standard staining procedures. Immunofluorescence staining for vitronectin (A), ApoE (B), TIMP3 (C), ApoJ (D), CFI (E), CFH (F), MITF (G), and RPE65 (H) are shown as well as appropriate secondary controls (I–K). Scale bars approximately 10um.
