Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 17;5:1102. doi: 10.1038/s42003-022-04082-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a, b Shutoff activity of Cal and Nan mutant PA-Xs. 293 T cells were transfected with expression plasmids for Cal PA-X a or Nan PA-X b with the indicated mutations along with pCAGGS firefly luciferase. Luciferase activity was determined by the Dual-Luciferase Reporter Assay system. c–e Translation efficiency of mRNAs produced by vRdRps containing PA frameshift mutant. 293 T cells were transfected with expression plasmids for Nan NP, PB1, PB2 and Cal PA FS (frameshift) or Nan PA FS with the indicated mutations along with pPolI-NP-luc. c Luciferase mRNA was quantified by qRT-PCR. d Luciferase activity was determined by the Dual-Luciferase Reporter Assay system. e Ratio of luciferase activity per mRNA, transformed to a normal distribution. Error bars show means plus/minus the standard deviations (n = 3 biological replicates). One-way ANOVA followed by Tukey’s multiple comparison test (*P < 0.05, **P < 0.01, ***P < 0.001).